Progress of multidisciplinary treatment of intrahepatic cholangiocarcinoma / 国际外科学杂志

Intrahepatic cholangiocellular carcinoma (ICC) has become the second common malignant tumors of the liver,with its incidence being next only to hepatocellular carcinoma (HCC).Due to the lack of targeted screening methods and specific clinical manifestations,most intrahepatic cholangiocarcinoma (ICC) is clearly diagnosed as advanced,which brings great difficulties to the treatment of ICC.At present,radical surgical resection is still the most effective method to ICC.However,for some patients who had a positive-margin (R1) resection or positive lymph node metastasis,multidisciplinary comprehensive treatment including transcatheter arterial chemoembolization (TACE),local ablation,systemic chemotherapy,targeted therapy and immunotherapy,etc.can effectively improve the prognosis of these patients.

Progress of multidisciplinary treatment of intrahepatic cholangiocarcinoma / 国际外科学杂志

Intrahepatic cholangiocellular carcinoma (ICC) has become the second common malignant tumors of the liver, with its incidence being next only to hepatocellular carcinoma (HCC). Due to the lack of targeted screening methods and specific clinical manifestations, most intrahepatic cholangiocarcinoma(ICC)is clearly diagnosed as advanced, which brings great difficulties to the treatment of ICC. At present, radical surgical resection is still the most effective method to ICC. However, for some patients who had a positive-margin (R1) resection or positive lymph node metastasis, multidisciplinary comprehensive treatment including transcatheter arterial chemoembolization (TACE), local ablation, systemic chemotherapy, targeted therapy and immunotherapy, etc. can effectively improve the prognosis of these patients.

Reconstruction of defect after resection of hypopharyngeal and cervical esophageal cancer by multiple tissue flaps / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To explore a new method for the reconstruction of defect after resection of hypopharyngeal and cervical esophageal cancer using pulled-up stomach combined with free jejunal flap or lanryngotracheal flap if pulled-up stomach can not reach the level of oral pharynx for ananstamosis.</p><p><b>METHODS</b>From June 2010 to June 2014, 56 cases of hypopharyngeal or cervical esophageal cancer were treated with pharyngogastric anastamosis, in 5 cases of them, because the length of pulled-up stomach was limited and could not reach oral pharynx, free jejunal flap was used in 2 cases with laryngeal invasion and laryngotracheal flap was used in 3 cases without laryngeal invasion to reconstruct the defect between oral pharynx and stomach.</p><p><b>RESULTS</b>Pharyngeal fistula occurred in 1 case with laryngotracheal flap reconstruction, but healed after 2 weeks of wound dressing. Other 4 cases had oral liquid diet two weeks after surgery and did not occur any complications such as infection or pharyngeal fistula. Follow-up showed 1 case died from mediastinal and lung metastases after 3 years, 1 case had cervical lymph recurrence after 2 years and still survived, and other 3 cases were tumor free survival for 28, 37, and 56 months respectively.</p><p><b>CONCLUSIONS</b>The defect after resection of hypopharyngeal and esophageal cancer can be reconstructed with pulled-up stomach combined with free jejunal flap or lanryngotracheal flap if pulled-up stomach can not reach the level of oral pharynx for ananstamosis.</p>

Cloning and analysis of promoter of pig copper zinc superoxide dismutase gene (CuZnSOD) / 生物工程学报

Pig copper zinc superoxide dismutase (CuZnSOD) is an important antioxidant enzyme. Some studies focused on the function of CuZnSOD gene, but the transcriptional regulation of the CuZnSOD gene is not yet fully elucidated. Therefore, the aims of the study were to determine the core promoter region and to explore its mechanism of transcriptional regulation. The 853 bp DNA sequence of 5'-flanking promoter was amplified by performing PCR. A series of CuZnSOD promoter fragments with gradually truncated 5'-end were produced by nested PCR and inserted into pGL3-Basic vector. The activities of the promoters were measured by the dual-luciferase assay system after transient transfection into the NIH/3T3 cells. The results demonstrated that there were 2 potential transcription start sites in the regions from initiation codon to -87 bp and -266 bp, respectively. The region from -383 bp to +67 bp in CuZnSOD gene promoter showed higher activity than other regions, and further deletion analysis demonstrated that the region from -75 bp to -32 bp contained an essential promoter sequence for pig CuZnSOD gene transcription. In addition, several potential transcription factor binding sites were predicted with bioinformatics method. These results suggest that these transcription factor binding sites may be involved in the transcriptional regulation of CuZnSOD gene.

Generation of transgenic rabbits expressing swine PID1 by testicular injection / 中国实验动物学报

Objective The aim of this study was to investigate the relationship between phosphotyrosine interac-tion domain containing 1 ( PID1 ) gene and variation in intramuscular fat ( IMF ) content and the possibility to generate transgenic animals by testicular injection .Methods Expression vector pIRES2-acGFP-PID1 carrying pig PID1 gene was incubated with transfection reagents and injected into the testes of male New Zealand rabbits .We examined the F1 genera-tion by fluorescence detection , PCR, Western blotting and measuring the IMF content .The F1 generation gave birth to the F2 generation.Then we examined the F2 generation through detecting the positive rate and the IMF content .Results The exogenous PID1 gene and fluorescent protein gene were expressed at different levels both in the F 1 generation and the F2 generation, and the positive rates were 35.88%and 34.33%, respectively.The IMF content was significantly elevated (P<0.05) in the transgenic positive individuals compared with the negative ones and the control group , and the PID1 protein expression was similarly higher .Conclusions The results of this study demonstrate that PID 1 gene affects intramuscular fat content significantly .Moreover, the results of our analysis provide further evidence that transgenic animals can be gener -ated by testicular injection , and the exogenous gene can be inherited steadily .